Hier werden Diplomarbeiten, auch externe, gepostet.

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Thomas Mansberger

"Gelassenheit ist eine anmutige Form des Selbstbewusstseins" (Marie Ebner von Eschenbach)

Thema der Diplomarbeit:
Charakterisierung der beim Restabbau von Pflanzenöl und PAKs beteiligten Mikroorganismengruppen und deren Aktivität - Adaptationsfähigkeit der mikrobiellen Gemeinschaftsstruktur auf wiederholte Pflanzenölausbringung

im Rahmen des Projektes: Entwicklung eines pflanzenöl-basierenden Verfahrens zur In-Situ-Sanierung von PAK-kontaminierten Böden

Methoden:
Inkubationsversuche - Abbauversuche
Phospholipidfettsäurenanalytik (GC-FID)
Isotopenanalytik (13C) (GC-c-IRMS)

Forschungsbeihilfe: 440 Euro / Monat

Beginn: Frühjahr 2007
Dauer: ca. 6 Monate

Ansprechpersonen:
Dr. Andrea Watzinger, Tel.: 01 47654 3109, andrea.watzinger@boku.ac.at
Prof. Martin Gerzabek, Tel.: 01 47654 3102, martin.gerzabek@boku.ac.at

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Thomas Mansberger

"Gelassenheit ist eine anmutige Form des Selbstbewusstseins" (Marie Ebner von Eschenbach)

Stellenbeschreibung:
Diploma student
Fachgebiet: Molecular and Cellular Leukemia Research
Forschungsstätte: Children's Cancer Research Institue, St. Anna Kinderkrebsforschung
E-Mail: rente.panzer@ccri.at
Kontaktperson: Univ. Prof. Dr. Renate Panzer
Webadresse: http://ccri.at

Ausschreibungstext:

We are looking for a highly motivated diploma student for Leukemia research on the topic:
Collaborating events in TEL-AML1 positive acute lymphoblastic leukemia.

This project is part of the research aiming at the identification of molecular mechansims in leukemia development in the context of projects supported by FWF and GENAU-CHILD.

The research in our laboratory focuses on the etiology and molecular pathogenesis of childhood acute lymphoblastic leukemia (ALL) aimed to improve our understanding of leukemia development. Our current major emphasis is on two aspects of ALL carrying the t(12.21) chromosomal translocation: The function of the TEL-AML1 fusion gene (also known as ETV6-RUNX1) and the role of potential secondary events in leukemia development. These studies are carried out in model systems by loss and gain of function studies and are validated in primary leukemic cells as well as in animal models. The results should lead to the elucidation of mechanisms by which the fusion genes/mutations exert their specific function and the information whether the particular fusion gene or its downstream effectors can be specifically targeted.

Requirements: highly motivated master student in molecular biology/genetic,laboratory experience in molecular biology advantageous, excellent team player with the ability to work independently.

Begin: 1/2007

We offer: motivating scientific environment, weekly lab meetings, journal clubs, and seminars. subsidized cafeteria, financial support.
Please send your application including a short CV to: Univ. Prof. Dr. Renate Panzer-Grümayer (renate.panzer@ccri.at)

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Thomas Mansberger

"Gelassenheit ist eine anmutige Form des Selbstbewusstseins" (Marie Ebner von Eschenbach)

Diplomand/in
ARC Seibersdorf research GmbH
Diplomarbeit: Entwicklung neuartiger bioaffiner Sensoroberflächen
http://alumni.boku.ac.at/jobs/job_show_ ... p?id=25410

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Thomas Mansberger

"Gelassenheit ist eine anmutige Form des Selbstbewusstseins" (Marie Ebner von Eschenbach)

Die Andritz-Gruppe mit Sitz in Graz, Österreich, ist ein weltweit tätiges Technologie-Unternehmen. Die
Gruppe beschäftigt rund 10.000 Mitarbeiter und verfügt über 50 Produktions- bzw. Servicestätten sowie
über 120 Tochtergesellschaften und Vertriebsniederlassungen auf der ganzen Welt. Für unseren weltweit
agierenden Geschäftsbereich MECHANICAL PULPING SYSTEMS unserer Wiener Niederlassung
(12. Bezirk) suchen wir eine/n qualifizierte/n

DIPLOMANDEN/IN
(Verfahrenstechnik)
Thema: Untersuchung von Aschegehalt (Sand) an
verschiedenen Prozessstellen von Holzstoff-Faserlinien

Aufgabe:
- Erhebung der Daten bei mehreren europäischen
Papierfabriken (Mittel- und Nordeuropa)
- Probenahme an den zu definierenden Prozesspunkten
- Aschegehaltbestimmung der Proben im Labor der
jeweiligen Fabrik
- Erhebung von Prozess-/Betriebsdaten an Hand von
vorbereiteten Check-Listen
- Zusammenfassung der Ergebnisse und Auswertung der

Ergebnisse
Zeitrahmen: Beginn ab sofort.
Das Ziel ist es, die Untersuchungen mit Sommer 2007 abzuschließen.

Sprache der Diplomarbeit: Englisch bevorzugt.

Aufwandsentschädigung/Bezahlung: Details werden
entsprechend der Andritz Richtlinien vereinbart.
Betreuer: Herr DI Johann Grossalber

Ihre Bewerbung: Bitte senden Sie Ihre aussagekräftigen
Bewerbungsunterlagen (Lebenslauf, Schul- bzw.
Studienzeugnisse, Foto) an nebenstehende Adresse.
Andritz AG
Eibesbrunnergasse 20
A-1120 Wien
z. H. Hr. DI Johann Großalber
In Vertr.: Fr. Mag. Sandra Purkert
FAX: +43-1/81195 96243
johann.grossalber@andritz.com
sandra.purkert@andritz.com
Internet: DIPLOMANDEN/IN www.andritz.com
(Verfahrenstechnik)

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Thomas Mansberger

"Gelassenheit ist eine anmutige Form des Selbstbewusstseins" (Marie Ebner von Eschenbach)

Intercell AG

DIPLOMA STUDENT
Position available from now on!!!

Title: Characterization of protective pneumococcal antigens for their role in the bacterial life cycle and pathogenesis
Project leader: Eszter Nagy

Background: Intercell AG is a successful biotechnology company focusing on vaccine development against numerous pathogens using platform technologies for genomic scale antigen identification and novel adjuvants. Streptococcus pneumoniae (Pneumococcus) is a widespread human pathogen that causes a variety of infections, many of those are life-threatening diseases such as pneumonia, bacteremia and meningitis. Despite the availability of licensed vaccines and antibiotic treatments, the morbidity and mortality attributed to S. pneumoniae remain significant in both developed and developing countries. At Intercell Pneumococcus is a target for the development of a protein-based vaccine and a humanized monoclonal antibody therapy for the treatment and the prevention of life threatening infections. Antigen identification and in vitro validation have been completed, and protective antigens are currently being prepared for human clinical trials. A research project that is ongoing already (PhD thesis) aims the better understanding of the role of these proteins in pneumococcal pathogenesis and host-parasite interaction. Based on gene deletion studies the two lead vaccine candidates have important non-redundant functions in bacterial multiplication showing reduced growth in vitro, abnormal septum formation and atypical cell shapes and lack of virulence in vivo.

Aim: Characterization of two pneumococcal antigens that are selected for vaccine development regarding their function in the bacterial cell cycle and their role in pathogenesis.
Methods:
Protein-protein interaction studies: Two Hybrid System, biochemical assays with recombinant proteins and cultures cell lines, binding studies, generation of knock-out strains
Cell cycle studies: localization using immunofluorescence and ELMI
Pathogenesis: in vivo studies of virulence and disease

For more information contact:
Eszter Nagy, MD, PhD ENagy@intercell.com, 01-20620-115
Carmen Giefing, CGiefing@intercell.com, 01-20620-231

Related publications:
[size=7]Giefing, C., M. Hanner, T. Henics, D.B. Minh, D. Gelbmann, U. Lundberg, B.M. Senn, M. Schunn, A. Habel, B. Henriques-Normark, A. Ortqvist, M. Kalin, A. von Gabain, A. Meinke, and E. Nagy. 2007. Discovery of a novel class of highly conserved vaccine antigens using genomic scale antigenic fingerprinting of Pneumococcus with human antibodies. J Exp Med [in review]

Giefing, C., E. Nagy, and A. von Gabain. 2007. The Antigenome: From Protein Subunit Vaccines to Antibody Treatments of Bacterial Infections. In Pharmaceutical Biotechnology. C.A. Guzman and G. Feuerstein, editors. Landes Bioscience, Austin, TX. In press.

Dryla, A., B. Hoffmann, D. Gelbmann, C. Giefing, M. Hanner, A. Meinke, A.S. Anderson, W. Koppensteiner, R. Konrat, A. von Gabain, and E. Nagy. 2007. High-affinity binding of the staphylococcal HarA protein to haptoglobin and hemoglobin involves a domain with an antiparallel eight-stranded beta-barrel fold. J Bacteriol 189:254-264.

Nagy, E., T. Henics, A. von Gabain, and A. Meinke. 2005. Identification of the 'Antigenome' - a novel tool for design and development of subunit vaccines against bacterial pathogens. In Genomics, Proteomics and Vaccines. G. Grandi, editor. John Wiley & Sons, Ltd. 223-243.

Meinke, A., T. Henics, and E. Nagy. 2004. Bacterial genomes pave the way to novel vaccines. Curr Opin Microbiol 7:314-320.

Dryla, A., D. Gelbmann, A. von Gabain, and E. Nagy. 2003. Identification of a novel iron regulated staphylococcal surface protein with haptoglobin-haemoglobin binding activity. Mol Microbiol 49:37-53.

Etz, H., D.B. Minh, T. Henics, A. Dryla, B. Winkler, C. Triska, A.P. Boyd, J. Sollner, W. Schmidt, U. von Ahsen, M. Buschle, S.R. Gill, J. Kolonay, H. Khalak, C.M. Fraser, A. von Gabain, E. Nagy, and A. Meinke. 2002. Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus. Proc Natl Acad Sci U S A 99:6573-6578.

Etz, H., D.B. Minh, C. Schellack, E. Nagy, and A. Meinke. 2001. Bacterial phage receptors, versatile tools for display of polypeptides on the cell surface. J Bacteriol 183:6924-6935.[/size]

6 Monatige Dipl Arbeit im Bereich Tumorforschung mit Unterstützung von Micro Arrays
Option auf Dissertation
genaue Beschreibung im pdf

Prof Glößl hat mir eine Ausschreibung von 37 PhD Positionen in der "Faculty of Agricultural Sciences, University of Aarhus" gesendet.
pdf im Anhang
Grüße Alf

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Prof Kneifl hat mir folgende Angebote weitergeleitet

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Und noch eine

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Liebe Leut ich hab wieder was bekommen also wer interesse hat oder jemanden kennt, einfach melden.


LG Alf

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Diplom-/Masterarbeiten im Rahmen des Forschungsprojektes BOKUfirst in Zusammenarbeit mit Baxter:

Das Programm BOKUfirst (females in research and technology) bietet BOKU-Studentinnen durch gezielte Zusatzqualifikationen eine Vorbereitung auf eine berufliche Karriere in F&E- und technologie-intensiven Unternehmen.

Genauere Infors:

http://www.boku.ac.at/bokufirst-ausschreibung.html

http://www.boku.ac.at/bokufirst.html



Themen:
http://www.boku.ac.at/bokufirst-themenboerse.html

Bewerbung mit Lebenslauf und aussagekräftigem Motivationsschreiben bitte bis 3. Mai 2009 an:
DI Roland Dunzendorfer (roland.dunzendorfer@boku.ac.at)

LG Kathi

_________________
ÖH-BOKU
Studienvertretung
Lebensmittel- und Biotechnologie

Dissertation:
Improvement of cellular productivity in recombinant CHO cells using flow cytometry and cell sorting

Objective:
• to find new strategies to use the cell sorter to quickly and efficiently isolate the highest producing subclones from a pool of transfectants
• to evaluate existing and new sorting methods

Currently existing methods to sort for high producers:
Cold Capture Surface Staining:
• a simple method, applicable to most products, however, does not always give a good correlation between the specific productivity and the fluorescent signal
Affinity matrix Surface staining:
• Reliable, but complicated (3 consecutive steps required to fix matrix onto cell surface)

New, to be developed methods:
New Affinity Matrix method
• Reduction of matrix fixation to a single step
Electroporation of cells using product specific labelled antibodies
• Electroporation allows to introduce not only plasmids, but also proteins into cells. Labelled product specific antibodies are retained only in cells that contain product
Amount of Endoplasmic Reticulum in the cell
• Plasma cells have been shown to have a significantly increased volume of ER, a similar increase in ER volume was sometimes observed in high-producer cells
Overall Protein Secretion rate
• A protocol exists that measures vesicle transport from the Golgi to the cytoplasma membrane thus giving an estimate of total secreted protein



If you are interested, please contact Nicole Borth, IAM 3rd floor, 36006 6232 or nicole.borth@boku.ac.at

Diplomarbeit:
Establishment and evaluation of cell lines with optimised
growth behaviour by directed evolution and cell sorting

The economic output of a biotechnological production processes is determined by the growth properties of the used cell line, the final achieved cell density as well as by its specific production rate. Mammalian cells, compared to recombinant bacteria and yeast, are notoriously slow and grow only to limited cell densities. We have recently established a directed evolution protocol assisted by flow-cytometric cell sorting that enabled the improvement of the final cell density and viability of a CHO host cell line, while at the same time reducing the glutamine dependency of the cells. Currently, the new host cell line is able to grow to higher final cell densities with extended viability compared to the parent cell line, but in the complete absence of glutamine in the culture medium. Glutamine is the main energy substrate of immortalised mammalian cells in culture, however, it is the source of ammonium, which causes a decrease in the sialidation of protein glycosylation. As the main reason for using mammalian cells is the fact that some proteins require the species-specific glycosylation patterns for biological activity, long in-vitro half life and low immunogenicity and as most of these parameters are dependend on terminal sialidation of the glycosylation chains, it is especially important to avoid high levels of glutamine in a production process.

The goal of the present master´s thesis will be to establish a production cell line growing in glutamine-free medium using the already established directed-evolution-protocol, characterisation of growth and viability of the resulting cell lines and evaluation of changes in productivity and in product quality (glycosylation)



If you are interested, please contact Nicole Borth, IAM 3rd floor, 36006 6232 or nicole.borth@boku.ac.at

Hi!

Würd ca. ab Jänner eine Diplomarbeit suchen.
Bevorzugt in den Bereichen Mobi, Virologie, QM,.... bei einer Firma zumindest mit angemessenem "Taschengeld" wäre optimal....

Leider tut sich hier schon lang nix mehr.

Hat irgendjemand Infos?

Vielen Dank im Voraus.