Despite compliance with current hygiene standards and the use of state-of-the-art concepts for cleaning and disinfection, the formation of bacterial biofilms is inevitable in the food industry. So far, there are no standardised methods to evaluate the cleaning efficacy on-site. One approach is the use of biofilm imitations. As part of the BioMitate2 project, the aim of this thesis is to develop new reproducible imitations and to characterise them accordingly in terms of cleaning relevant properties.