Enthusiastic lab members wanted!

 

 

Post Doc, Dissertation(s), Technician

 

Improvement of Chinese Hamster Ovary Cell CHO platform technology: Cell line optimisation and engineering

 

Objective:

• Development of tunable gene expression toolbox


• Establishment of a stable host cell line


• Controlled induction of a high productivity phenotype

 

Current problems in mammalian cell line development

·        Cell lines are phenotypically and physiologically unstable due to high number of  chromosomal rearrangements

·        Host cell optimisation difficult, as properties are lost upon transfection

·        Random transfection induces more uncontrollable genomic changes

·        Labour and time intensive screening required

·        Development times for cell lines: 4-8 months

 

To do´s

·        Host cell line with higher genomic stability

·        Targeted integration toolbox

·        Control over cellular properties

 

Wanted

·        Enthusiastic people, who want to work in a group at the forefront of CHO-research

·        Independent thinkers who like to discuss new ideas and work on their realisation

·        Interested individuals who want to learn cell culture technology, bioinformatics and cell engineering

 

 Start January 2012

 

If you are interested, please contact Nicole Borth, DBT 3^rd floor, 47564 6232 or nicole.borth@boku.ac.at